| ADH;VP;AVP

Vasopressin, also called antidiuretic hormone (ADH), arginine vasopressin (AVP) or argipressin,is a hormone synthesized from the AVP gene as a peptide prohormone in neurons in the hypothalamus,and is converted to AVP. It then travels down the axon terminating in the posterior pituitary, and is released from vesicles into the circulation in response to extracellular fluid hypertonicity (hyperosmolality). AVP has two primary functions. First, it increases the amount of solute-free water reabsorbed back into the circulation from the filtrate in the kidney tubules of the nephrons. Second, AVP constricts arterioles, which increases peripheral vascular resistance and raises arterial blood pressure.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	500	250	125	62.5	31.25	15.62	7.81	0
OD	0.188	0.27	0.389	0.642	0.929	1.38	1.766	1.842

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse ADH;VP;AVP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse ADH;VP;AVP and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-108	105-115
	Average (%)	93	98	110